【论著】自然流产小鼠绒毛滋养细胞PCNA和OPN的表达与相关研究

09-06- 12

摘要目的比较正常妊娠和自然流产小鼠绒毛滋养细胞增殖细胞核抗原（Proliferating Cell Nuclear Antigen, PCNA）和骨桥蛋白（Osteopontin, OPN）的表达，探讨自然流产的发病机制。方法建立正常妊娠模型CBA×BALB/c和自然流产模型CBA×DBA/2。采用免疫组化SP法测定两组孕13 d绒毛滋养细胞PCNA和OPN的表达。结果与正常妊娠相比，自然流产绒毛滋养细胞PCNA和OPN的表达均明显降低（P<0.01，P<0.05）。结论绒毛滋养细胞PCNA和OPN的表达低下与自然流产的发生有关。

关键词自然流产绒毛滋养细胞增殖细胞核抗原骨桥蛋白 Expressions of PCNA and OPN in trophoblasts of spontaneous abortion mouse model and the relevant research QIN ChenMI YayingYANG WeibinLIU Lihua ABSTRACTObjectiveTo investigate the relationship of spontaneous abortion and the expressions of proliferating cell nuclear antigen (PCNA) and osteopontin（OPN） in trophoblasts of spontaneous abortion mouse models. MethodsThe nonabortionprone CBA×BALB/c matings were established as the models of normal pregnancy and the abortion prone(CBA×DBA/2) matings were used as the models of spontaneous abortion. Immunohistochemistry method(SP) was employed to detect the expressions of PCNA and OPN in trophoblasts at the day 13 of gestation. ResultsCompared with normal pregnancy models, both of the expressions of PCNA and OPN in trophoblasts of spontaneous abortion mouse models were significantly decreased (P<0.01,P<0.05). ConclusionThe weakening of PCNA and OPN in trophoblastic cells may be related to the occurrence of spontaneous abortion. KEY WORDSSpontaneous abortionTrophoblastsProliferating cell nuclear antigen (PCNA) Osteopontin（OPN）

作者单位1. 山西医科大学大同学院免疫与病原生物研究所 2. 大同市红十字会中心血站