副主任：吴超群 教授
07-09-25
吴超群 教授
1945年11月出生，医学博士，复旦大学生命科学学院，遗传工程国家重点实验室教授。上海细胞生物学学会理事、国际细胞因子协会资深会员。
1968年毕业于上海医科大学医学系， 1991年公派赴美国克利夫兰医学中心任博士后研究员，1997在澳大利亚悉尼新南威尔士大学病理系任客座研究员，1998年11月回国至今在复旦大学生命科学院遗传所，遗传工程国家重点实验室工作。长期从事医学分子生物学和细胞生物学研究，主要涉及分子细胞生物学教学和细胞分化的表观遗传学和基因表达谱，环境和营养因素对人体基因的表观遗传学调控等方面的研究，人体巨噬细胞特异性基因的克隆、表达及相应功能研究，一氧化氮合成酶蛋白的晶体结构分析及功能的研究，人血小板造血因子(TPO, thrombopoitin)诱导的造血细胞内NFkappaB信号途径研究。
1999年起承担或共同承担浙江省自然科学基金课题，国家自然科学基金面上和重点项目等五项。曾获2002年教育部二等奖，2002年上海市科技成果证书，2001年浙江省中医药科技成果一等奖。1988年卫生部科技进步二等奖和1988年卫生部科技进步二等奖和1987年浙江省科技进步二等奖。培养博士研究生3名，硕士研究生5名。发表研究论文38篇，其中Sci 杂志27篇。任中国大百科全书（第二版）遗传学科副主编，主编出版著作“细胞生物学英语教材”，“表观遗传学原理与应用”两部，译著“与您的基因对话?遗传营养工程学”和表观遗传学常用技术” 两部。 参编“生物化学技术原理及应用”，。参译“基因VIII”，“遗传学原理”等三部。
发表的主要论文
1. Julie M.Tebo, Chaoqun Wu, Yoshihiro Ohmori, and Thomas A.Hamilton: Murine Inhibitory Protein-kappa B Alpha Negatively Regulates Kappa B-dependent Transcriptionin Lipopolysaccharide-stimulated RAW 264.7 Macrophage. Journal of Immunology(USA), 153:4713,1994 Nov 15
2. Chaoqun Wu, Yoshihiro Omori, Sudip Bandyopdhyay, Ganes Sen, and Thomas Hamilton: Interferon-
Stimulated Response Element and NF?B Sites Cooperate to Regulate Double-Straned RNA-Induced
Transcription of IP-10 Gene, Journal of Interferon Research(USA) 14:357-363, 1995
3.Chaoqun Wu, Wanleng Deng, Yoshihiro Ohmori, and Thomas A. Hamilton:DifferetialMechanisms of LPS-induced NF-kB Activation in Macrophages and Fibroblasts, Journal of Endotoxin Research(USA) 3(1), 9-18, 1996
4. Uma Siddhanta, Chaoqun Wu, Husam M. Abu-Soud, Jingli Zhang, Dipak K. Ghosh, and Dennis J. Stuehr: Heme Iron Reduction and Catalysis by a Nitric Oxide Synthase Heterodimer Containing One Reductase and Two Oxygenase Domains, Journal of Biochemistry and Chemistry(USA) 271 (13): 7309, 1996.
5.Chaoqun Wu, Jingli Zhang, Husam Abu-Soud, Dipak K. Ghosh, and Dennis J. Stuehr: High-level Expresion of Inducible Nitric Oxide Synthase in E.Coli Requires Coexpression with Calmodulin, BBRC(USA) 222, 439-444，1996
6.R.Gachhui, D.K. Ghosh, Chaoqun Wu, J. Parkison, B.R. Crane and D.J. Stuehr: Mutagenesis of Acidic Residues in the Oxygenase Domain of Inducible Nitric Oxide Synthase Identifies A Glutamate Involved in Arginine Binding, Biochemistry(USA) 36: 5097-5103, 1997
7.B.Mayer, Chaoqun Wu, A.C. Gorren, S. Pfeiffer, K. Schmidt, P. Clark, D.J. Stuhehr & E.R.Werner.: Tetrahydrobiopeterin Binding to M crophage Inducible Nitric Oxide Synthase:Heme Spin Shift and Dimer Stailization by the Potent Peterin Antagonist 4- Ammino- tetrahydrobiopterin. Biochemistry(USA) 36: 8422-8427, 1997
8.Chaoqun Wu, D.K. Ghosh E. Pitters, M. Molony, E.R. Wener, B. Mayer & D.J. Stuehr, : Characterization of the Inducible Nitric Synthase Oxygenase Domain Identifies a 49 Amino Acid segment required for Subunit Dimerization and tetrahydrobiopetrin Interaction. Biochemistry(USA) 36: 10609 - 10619, 1997
9.B.R. Crane, A.S. Arvai, Chaoqun Wu, D.K. Ghosh, E.D. Getzoff, D.J. Stuehr & J.A. Tainer : The Structure of Nitric Oxide Synthase Domain and Inhibitor Complexes. Science (USA) 278 : 425 - 431, 1997
10. Brian R. Crane. Andrew S. Arvai, Dipak K. Ghosh, Chaoqun Wu, Elizabeth D. Getzoff, Dennis J. Stuehr, John A. Tainer Structure of Nitric Oxide Synthase Oxygenese Dimer with Pterin and Substrate Science(USA) 279:2121-212, 1998
11. Husam M. Abu-Soud, Chaoqun Wu, Dipak K. Ghosh, and Dennis J. Stuehr Stopped-Flow Analysis of CO and NO Binding to Inducible Nitric Oxide Synthase Biochemistry 37(11):3777-3786. 1999.
12. Ruilan Gao ，Chaoqun Wu：Hematopoietic Signaling induced by Saponins of Panax Ginsen, Chinese Journal of Hematology, 20（6）292-295, 1999
13. Ruilan Gao ，Chaoqun Wu: Saponins of Panax Ginsen induced Megakaryocytic Differentiation through GATATranscriptional Factors, Chinese Journal of Clinic Hematology 12（2）， 50-54,1999
14. Chaoqun Wu, Weiping Liu, Youzhong Yuan, Rong Tang, Yi Xie, and Yumin Mao : Gene Expression Pattern of Early Respons in Endothelials treated with PMA. Acta of Second Millitary Medical University, 22( 9 ): 479, 2000
15. Yao Li, Mingyan Qiu, Chaoqun Wu, Weiping Liu, Youzhong Yuan, Rong Tang, Yi Xie, and Yumin Mao: Differential Expression between Hepatocellular Carcinoma and Hepatic Cells. Acta Genetics.27（12）：427， 2000
16. Shengrong Lin, Alexander G. McLennan, Kang Ying, Zhao Wang, Shaohua Gu, Hua Jin, Chaoqun Wu, Yi Xie, and Yumin Mao: Cloning, Expression, and Characterization of a Human Inosine Triphosphate Pyrophosphatase Encoded by the ITPA Gene。 J. Biol. Chem. 2001 276: 18695-18701.
17. Zhen Zhang, Chaoqun Wu, Shu Wang, Wei Huang, ZongXiang Zhou, Kang Ying, Yi Xie, YuMin Mao. Cloning and Characterization of ARHGAP12, a novel human rhoGAP gene. International Journal of Biochemistry and Cell Biology 2002 Apr;34(4):325-31
18. Zhen Zhang, Chaoqun Wu, Wei Huang, Shu Wang, Yi Xie, YuMin Mao. A novel human gene whose product shares homologue with bovine brain-specific protein p25 is expressed in fetal brain but not in adult brain. Journal of Human Genetics 2002 May;47(5):266-8
19. Quoqian Wang，Chuipin Zhong, Jidong Fu, Qian Fan, Chaoqun Wu： Specific Cytotoxicity of T lymphocyte Mediated by DCs treated with Total mRNA from Hepatocancer, Journal of Chinese Immunology,（18）2：102-105, 2002
20. Liu SL, Lin X, Shi DY, Cheng J, Wu CQ, Zhang YD: Reactive oxygen species stimulated human hepatoma cell proliferation via cross-talk between PI3-K/PKB and JNK signaling pathways. Arch Biochem Biophys. 2002 Oct 15;406(2):173-82
21. Qi ZY, Li Y, Ying K, Wu CQ, Tang R, Zhou ZX, Chen ZP, Hui GZ, Xie Y: Isolation of novel differentially expressed genes related to human glioma using cDNA microarray and characterizations of two novel full-length genes. J Neurooncol. 2002 Feb;56(3):197-208
22. 李博，唐孝达，夏术阶，徐东亮，张新华，鲁军，吴超群，钟翠平：转染干扰素－γ诱导蛋白－10基因构建树突状细胞前列腺癌瘤苗及其抗肿瘤免疫作用的检测 中华实验外科杂志，2003，20（12）：1092～1094
23. 张新华、钟翠平、周播江、顾云娣、冯久贤、吴超群: 转化生长因子β1基因转染对大鼠骨髓树突状细胞免疫功能的影响 解剖学报 2004，35（2）：184～189
24. 核因子-kB对小鼠树突状细胞分化成熟及其体外刺激T细胞免疫反应的影响 徐东亮，唐孝达，李博，刘永，张新华，吴超群，钟翠平 中华器官移植杂志，2004，25（2）：89～92
25. Gao RL, Xu WH, Lin XJ, Chen XH, Wu CQ.; Up-regulation of transcription factors GATA-1 and GATA-2 induced by panax notoginosides in hematopoietic cells. Zhonghua Xue Ye Xue Za Zhi. 2004 May;25(5):281-4.
26. 干扰素－γ诱导蛋白－10基因修饰小鼠树突状细胞的抗前列腺癌作用研究 李博，唐孝达，徐东亮，张新华，鲁军，吴超群，钟翠平 中华泌尿外科杂志，2004，25（4）
27. Xu DL, Liu Y, Tan JM, Li B, Zhong CP, Zhang XH, Wu CQ, Tang XD: Marked prolongation of murine cardiac allograft survival using recipient immature dendritic cells loaded with donor-derived apoptotic cells. Scand J Immunol. 2004 Jun;59(6):536-44.
28. Gao RL, Xu WH, Chen XH, Qian XD, Wu CQ: Up-regulation of transcription factors NF-E2, c-jun and c-fos of AP-1 family induced by Panax Notoginosides in hematopoietic cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2004 Feb;12(1):16-19
29. 张新华,钟翠平,周播江,梁春敏,顾云娣,吴超群: 转化生长因子β1基因修饰树突状细胞延长移植心脏存活时间的研究. 现代免疫学,2004,24(6):464-469
30. Huirong Yang, Chaoqun Wu, Shouyuan Zhao, Jinhu Guo: Identification and characterization of D8C, a novel domain present in liver-specific LZP, uromodulin and glycoprotein 2, mutated in familial juvenile hyperuricaemic nephropathy. FEBS Letters, 2004, 578:236?238
31. 周播江,钟翠平,顾云娣,张新华,梁春敏,吴超群：肝再生大鼠血清诱导骨髓干细胞向肝细胞分化的实验研究 中华肝脏病杂志,2004,12(12):730
32. Yang H, Wu Chaoqun, Zhao S, Guo J.: Identification and characterization of D8C, a novel domain present in liver-specific LZP, uromodulin and glycoprotein 2, mutated in familial juvenile hyperuricaemic nephropathy. FEBS Lett. 2004 Dec 17;578(3):236-8.
33. Cao L, Yan X, Borysenko CW, Blair HC, Wu Chaoqun, Yu L.: CHDL: a cadherin-like domain in Proteobacteria and Cyanobacteria. FEMS Microbiol Lett. 2005 Oct 15;251(2):203-9.
34. Zhang K, Gao R, Zhang H, Cai X, Shen C, Wu Chaoqun, Zhao S, Yu L: Molecular cloning and characterization of three novel lysozyme-like genes, predominantly expressed in the male reproductive system of humans, belonging to the C-type lysozyme/alpha-lactalbumin family. Biol Reprod. 2005;73(5):1064-71.
35. Kexiong Zhang, Rui Gao, Haoxing Zhang, Xin Cai, Chunhua Shen, Chaoqun Wu, Shouyuan Zhao, and Long Yu: Molecular Cloning and Characterization of Three Novel Lysozyme-Like Genes, Predominantly Expressed in the Male Reproductive System of Humans, Belonging to the C-Type Lysozyme/Alpha-Lactalbumin Family. Biol Reprod, 2005; 73: 1064 ? 1071
36. Lihuan Cao, Xiaomei Yan, Christopher W. Borysenko b, Harry C. Blair c, Chaoqun Wu, Long Yu: CHDL: A cadherin-like domain in Proteobacteria and Cyanobacteria. FEMS Microbiology Letters, 2005, 251:203?209.
37. Huan Fang, Wei Huang, Yingying Xu, Shen Zonghou and Chaoqun Wu: Blocking of N-acetylglucosaminyltransferase V Induces Cellular Endoplasmic Reticulum Stress and the Activation of Caspase-12 in Hepatocarcinoma 7721 cells. Cell Research. (Accepted and expected Jan 2006)
38. Yu Kang, Cong-Jian Xu, Chao-Qun Wu, Xi-Shi Liu, Cui-Ping Zhong, Xin-Hua Zhang, Shou-Yi Qiao, Jian-Ren Gu: A novel strategy to compensate the disadvantages of live vaccine using suicide-gene system and provide better antitumor immunity. (Accepted and expected Jan 2006)
